> ## Documentation Index
> Fetch the complete documentation index at: https://docs.ospribrain.com/llms.txt
> Use this file to discover all available pages before exploring further.

# RDML Viewer

> Import and visualize qPCR RDML data files.

## Overview

The RDML Viewer imports standard RDML (Real-time PCR Data Markup Language) files and provides interactive visualization of qPCR data.

## Importing Data

### Upload RDML File

1. Navigate to the **RDML Viewer**
2. Click **Upload** and select your `.rdml` file
3. The file is parsed and data is displayed

### Load from Synthetic Data

You can also load data generated by the Synthetic Data Generator:

1. Go to Synthetic Data → History
2. Click **Load in RDML Viewer** on any run

## Analysis Tabs

| Tab                    | Description                                   |
| ---------------------- | --------------------------------------------- |
| **Data Visualization** | Plate layout and amplification curves         |
| **Quantization**       | Cq/Ct values and standard curve analysis      |
| **Gene Expression**    | Relative gene expression calculations         |
| **PC/QC**              | Positive control and quality control analysis |

## Plate Layout

Interactive well grid showing:

* Well positions (A1-H12)
* Cq/Ct values per well
* Sample name and target
* Dye/fluorophore

Click any well to view its amplification curve.

## Curves Viewer

Amplification curves display:

* Fluorescence intensity vs. cycle number
* Threshold line for Cq determination
* Color-coded by target or sample
* Zoom and pan controls

<Note>
  **Alex's screenshot note:** Take a screenshot of the RDML Viewer showing the plate layout on the left and amplification curves on the right for selected wells.
</Note>

## Quantization

* Automatic Cq/Ct determination
* Standard curve fitting (if standards included)
* Concentration calculations
* Efficiency metrics

## Gene Expression

* ΔΔCt relative quantification
* Reference gene normalization
* Fold-change calculations
* Statistical analysis